Data-Driven qRT-PCR Normalization Using NORMAgene R package NORMAgene version 0.1.1 Enables correction for technical variance in raw quantitative reverse transcription polymerase chain reaction (qRT-PCR) data using the least squares-based NORMAgene data-driven normalization algorithm originally described by Heckmann et al. (2011) <doi:10.1186/1471-2105-12-250>. Performs normalization of raw crossing threshold values (CT) and also calculates relative variability metrics that can be used to assess the impact of normalization on variance. Software Depends: R (>= 3.5) Suggests: testthat (>= 3.0.0) Grant C. O'Connell <goconnell.phd@gmail.com> Comprehensive R Archive Network (CRAN) Grant C. O'Connell [aut, cre] (ORCID: <https://orcid.org/0000-0002-2642-6670>) GPL-3 2026-02-28 application/tgz https://CRAN.R-project.org/package=NORMAgene doi:10.32614/CRAN.package.NORMAgene