Compositional Statistical Framework for RNA Fractionation Analysis R package FracFixR version 1.1.0 A compositional statistical framework for absolute proportion estimation between fractions in RNA sequencing data. 'FracFixR' addresses the fundamental challenge in fractionated RNA-seq experiments where library preparation and sequencing depth obscure the original proportions of RNA fractions. It reconstructs original fraction proportions using non-negative linear regression, estimates the "lost" unrecoverable fraction, corrects individual transcript frequencies, and performs differential proportion testing between conditions. Supports any RNA fractionation protocol including polysome profiling, sub-cellular localization, and RNA-protein complex isolation. Software Depends: R (>= 4.0.0) Imports: future.apply (>= 1.8.1), nnls (>= 1.4), ggplot2 (>= 3.3.5), dplyr (>= 1.0.7), RColorBrewer (>= 1.1-2), tidyr (>= 1.1.3), matrixStats (>= 0.60.0), aod (>= 1.3.1), parallelly (>= 1.30.0), stats, utils, future, grDevices Suggests: testthat (>= 3.0.0), knitr, rmarkdown Alice Cleynen <alice.cleynen@cnrs.fr> Comprehensive R Archive Network (CRAN) Alice Cleynen [aut, cre] (ORCID: <https://orcid.org/0000-0001-8083-0204>), Agin Ravindran [aut], Nikolay Shirokikh [aut] (ORCID: <https://orcid.org/0000-0001-8249-358X>) CC BY 4.0 2026-05-11 application/tgz https://CRAN.R-project.org/package=FracFixR doi:10.32614/CRAN.package.FracFixR