Axon Integrity Evaluation System for Microscopy Images R package AiES version 0.99.6 Provides tools for the quantitative analysis of axon integrity in microscopy images. It implements image pre-processing, adaptive thresholding, feature extraction, and support vector machine-based classification to compute indices such as the Axon Integrity Index (AII) and Degeneration Index (DI). The package is designed for reproducible and automated analysis in neuroscience research. Software Imports: EBImage, e1071, stats, fs, data.table Suggests: utils, stringr, colorspace, dplyr, knitr, tcltk, rstudioapi, svDialogs Shinji Tokunaga <tokunaga@ncnp.go.jp> Comprehensive R Archive Network (CRAN) Shinji Tokunaga [aut, cre] (ORCID: <https://orcid.org/0000-0001-8398-3054>), Masafumi Funakoshi [ctb], Toshiyuki Araki [ctb] (ORCID: <https://orcid.org/0000-0003-3625-2042>) BSD_3_clause + file LICENSE (https://CRAN.R-project.org/package=AiES/LICENSE) 2025-06-06 application/tgz https://CRAN.R-project.org/package=AiES doi:10.32614/CRAN.package.AiES